Anti-EpCAM antibody, Mouse monoclonal
SIGMA/SAB4200704 - clone Ber-EP4, purified from hybridoma cell culture
Synonym: Anti-CD326 antigen; Anti-EGP; Anti-Epithelial cell surface antigen; Anti-Epithelial glycoprotein; Anti-KS 1/4 antigen; Anti-KSA; Anti-Major gastrointestinal tumor-associated protein GA733-2; Anti-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human colon carcinoma sections with 5 μg/mL Monoclonal Anti-EpCAM Clone:Ber-EP4 (Cat. No. SAB4200704) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence MCF7 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 μg/mL Monoclonal Anti-EpCAM Clone:Ber-EP4 (Cat. No. SAB4200704). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Ber-EP4, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1-2 μg/mL using human breast adenocarcinoma MCF-7 cell line. |
| immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections. | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P16422  |
| Biochem/physiol Actions: | The epithelial cell adhesion molecule (EpCAM) is highly expressed in carcinomas. It regulates cell signalling, proliferation, migration and adhesion. Proteolytic cleavage of EpICD domain is crucial for tumor progression. EpCAM is highly expressed in breast cancer and is considered as a potential target for gene therapy. Deletions in the 3′ region of the EpCAM gene is associated with colorectal cancer based lynch syndrome. Single nucleotide polymorphism in EpCAM gene is associated with infant diarrheal disorder, congenital tufting enteropathy(CTE). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The epithelial cell adhesion molecule (EpCAM) is mapped to human chromosome 2p21 and has nine exons. EpCAM is a 40 kDa transmembrane glycoprotein present on cell surface. It has an intracellular domain called the EpICD, comprising of 26-amino acids. The extracellular domain (EpEX) has an epidermal growth factor (EGF) and a thyroglobulin repeat-like domain. |
| Immunogen: | human breast carcinoma cell line MCF-7 |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |