Anti-Neurofilament 200 (Phos and Non-Phos) antibody, Mouse monoclonal
SIGMA/SAB4200705 - clone N52, purified from hybridoma cell culture
Synonym: Anti-CMT2CC; Anti-NFH
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human cerebellum sections with 10 μg/mL Anti-Neurofilament 200 antibody, Mouse monoclonal, Clone: N52 (Cat. No. SAB4200705).The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole extract of SH-SY5Y cells was separated on SDS-PAGE and probed with Anti-Neurofilament 200 antibody, Mouse monoclonal, Clone: N52 (Cat. No. SAB4200705). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1.10 μg/mL antibody 2. 5 μg/mL antibody 3. 2.5 μg/mL antibody 4. Negative Control: without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | N52, monoclonal |
| concentration | ~1.0 mg/mL |
| isotype | IgG1 |
| mol wt | ~200 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, pig, feline, rat, monkey, bovine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 2.5-5 μg/mL using human neuroblastoma SH-SY5Y cell line fresh lysate |
| immunofluorescence: suitable | |
| immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Cerebellum sections | |
| UniProt accession no. | P12036  |
| Application: | Anti-Neurofilament 200 (Phos and Non-Phos) antibody has been used in • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-Neurofilament 200 (Phos. and NonPhos.) also known as Neurofilament-H or Heavy subunit, specifically recognizes an epitope on the C-terminal tail domain of Neurofilament 200, which is present on both the phosphorylated and nonphosphorylated forms of this glycoprotein. The antibody shows reactivity with neurofilaments in the central and peripheral nervous systems from human , pig, mouse, rat, monkey, feline and bovine origin. |
| Biochem/physiol Actions: | Mutation in the NEFH gene leads to the development of autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc). |
| Biochem/physiol Actions: | Neurofilament 200 has an important function in axonal transport, axonal plasticity and maintaining the structure of neuronal cytoskeleton. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Neurofilament 200 (phosphorylated and non-phosphorylated) (mouse IgG1 isotype) is derived from the N52 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilament 200 also known as Neurofilament heavy polypeptide (H-subunit) and is 200 kDa. |
| General description: | Neurofilament 200 is encoded by the gene located at human chromosome 22q12.2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |