Anti-TM4SF4 antibody, Mouse monoclonal
SIGMA/SAB4200706 - clone TM4SF-11, hybridoma cell culture supernatant
Synonym: Anti-ILTMP; Anti-il-TMP transmembrane 4 L six family member 4
Product Type: Chemical
Immunoblotting Cell lysates were separated on SDS-PAGE and probed with 1:10,000 Anti-TM4SF4 antibody, Mouse monoclonal, Clone: TM4SF-11 (Cat. No. SAB4200706). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HepG2; 2. A549; 3. 3T3; 4. Hepa1-6; 5. PC12; 6. COS7; 7. MDBK; 8. MDCK
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | TM4SF-11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgM |
| mol wt | antigen ~27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, canine, monkey, mouse, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1:4,000-1:8,000 using whole extract of HCT-116 cells |
| UniProt accession no. | P48230  |
| Application: | Anti-TM4SF4 antibody has been used in immunoblotting. |
| Biochem/physiol Actions: | TM4SF4 (Transmembrane 4 L6 Family member 4) can intervene density-dependent cell proliferation. TM4SF4 levels increases in non-dividing epithelial cells during their differentiation and migration of intestinal crypts. TM4SF4 levels are upregulated in 80% of hepatocellular carcinoma (HCC) tissues and in stomach adenocarcinoma cells. Reduction of TM4SF4 expression in HCC cell lines by stably transfecting TM4SF4 antisense plasmid caused significant inhibition of cell proliferation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-TM4SF4 antibody, Mouse monoclonal (mouse IgM isotype) is derived from the TM4SF-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with synthetic peptide from the internal region of human TM4SF4, conjugated to KLH. TM4SF4 (Transmembrane 4 L6 Family member 4) also known as ILTMP (intestine and liver tetraspan membrane protein) is a member of the transmembrane 4 superfamily (or tetraspanin family). It is a cell surface glycoprotein containing four transmembrane domains. |
| Immunogen: | synthetic peptide from the internal region of human TM4SF4 |
| Physical form: | The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |