Anti-Collagen Type IV antibody, Mouse monoclonal
SIGMA/SAB4200709 - clone col-94, purified from hybridoma cell culture
Synonym: Anti-col4a1/col4a2
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human tongue sections with 8 μg/mL Monoconal Anti-Collagen Type IV, Clone: col-94 (Cat. No. SAB4200709). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | col-94, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| electron microscopy: suitable | |
| immunohistochemistry: 4-8 μg/mL using formalin-fixed, paraffin-embedded human tongue sections | |
| UniProt accession no. | P02462  |
| Application: | Anti-Collagen Type IV antibody, Mouse monoclonal has been used in immunohistochemistry and dot-blotting. |
| Biochem/physiol Actions: | Collagen Type IV represents a physical barrier against cell invasion. Proteolysis/degradation of Collagen Type IV is essential for cell migration and cancer cells invasion through the ECM into the interstitial tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Collagen Type IV antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the col-94 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with human collagen Type IV. Collagen Type IV (also known as COL4A) is a key component of the basement membranes (BM), which is found at the basal surface of epithelial and endothelial cells and is essential for tissue strength and polarity. Type IV Collagens are encoded by three pairs of paralogous genes (COL4A1 through COL4A6). |
| Immunogen: | human collagen Type IV |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |