Anti-Calponin antibody, Mouse monoclonal
SIGMA/SAB4200710 - clone hCP, purified from hybridoma cell culture
Synonym: Anti-CNN-1; Anti-CNN-2
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human tonsil sections with 20 μg/mL Monoconal Anti-Calponin, Clone: hCP (Cat. No. SAB4200710). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoconal Anti-Calponin, Clone: hCP (Cat. No. SAB4200710). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. COS7; 2. MCF7; 3. MDA-MB-231; 4. CaCo2; 5. HS-68; 6. C2C12; 7. NIH-3T3; 8. Rat2; 9. CHO; 10. MDBK; 11. MDCK
Immunoblotting Whole extract of human uterus was separated on SDS-PAGE and probed with Monoclonal Anti-Calponin, Clone: hCP (Cat. No. SAB4200710). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. Negative control: without first antibody.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | hCP, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~34 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, monkey, human, rabbit, ferret, mouse, hamster, rat, canine, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human tonsil sections | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | Q99439  |
| Application: | Anti-Calponin antibody, Mouse monoclonal may be used in: • immunoblotting • immunoprecipitation • immunohistochemistry |
| Biochem/physiol Actions: | Calponin is an F-actin, calmodulin, and tropomyosin binding protein that presents in both the cytoskeleton and contractile machinery of smooth muscle (SM) cells. The interaction of Calponin with actin is shown to inhibit myosin Mg-ATPase activity. The binding strength of calponin to tropomyosin is weakened in the presence of Ca2+. Calponin is involved in a variety of cellular processes including smooth muscle contraction, cell movement, shape change, angiogenesis, exocytosis and endocytosis. Monoclonal anti-Calponin can be used for the identification and localization of calponin in SM elements. Calponin levels may serve as a prognostic factor in hepatocellular carcinoma, renal cell carcinoma, osteosarcoma and melanoma. |
| Biochem/physiol Actions: | Monoclonal Anti-Calponin specifically recognizes smooth muscle Calponin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Calponin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hCP hybridoma (also cites as CALP), produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Calponin (CNN) exists in three isoforms CNN1, CNN2 and CNN3. CNN2 gene is mapped to human chromosome 19p13.3. All three isoforms are expressed in smooth muscle. While CNN1 expression is confined only to smooth muscle, CNN2 and CNN3 are expressed in other cells including the neuronal tissue, fibroblast, myoblast and B lymphocyte. The three isoforms differ in the C-terminal region and comprise conserved actin-binding middle domain and a N-terminal calponin homology (CH) domain. |
| Immunogen: | human uterus smooth muscle extract |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |