Anti-β-tubulin antibody, Mouse monoclonal
SIGMA/SAB4200715 - clone 2-28-33, purified from hybridoma cell culture
Synonym: Anti-TUBB1
Product Type: Chemical
Immunofluorescence HS68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2 μg/mL Anti-β-Tubulin antibody, Mouse monoclonal,Clone: 2-28-33 (Cat. No. SAB4200715).The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Anti-β-Tubulin antibody, Mouse monoclonal, Clone: 2-28-33 (Cat. No. SAB4200715). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. MDBK; 2. HS68; 3. BE; 4. HeLa; 5. PC12; 6. 293; 7. MDCK; 8. COS7; 9. CFB; 10. 3T3; 11. L8; 12. HS68
Immunoblotting Lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Anti-β-Tubulin antibody, Mouse monoclonal, Clone: 2-28-33 (Cat. No. SAB4200715). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Rat Heart 2. Rat Testis 3. Rat Skeletal muscle
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2-28-33, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | dog, chicken, rat, bovine, monkey, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract |
| immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells | |
| immunohistochemistry: suitable | |
| UniProt accession no. | P07437  |
| Application: | Monoclonal Anti-β-tubulin has been used: • in immunoblotting • in immunofluorescence • in immunohistochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-β-tubulin specifically recognizes the two major β-tubulin isotypes and one of the minor β-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, neuronal axons of the larva of Ciona intestinalis (ascidian), microtubules in root tip cells in Arabidopsis thaliana and to human, mouse, rat, monkey, bovine, dog and chicken β-tubulin. The different β-tubulin isotypes are suggested to differ from each other in their ability to polymerize into microtubules. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-β-tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 2-28-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin also known as TUBB is the major building block of microtubules. Microtubules assemble from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of ~ 50 kDa and share considerable homology. For β-tubulin, five evolutionarily conserved isotypes have been identified, which are regularly utilized in the same cell types of different species. |
| Immunogen: | Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |