Anti-Vimentin antibody, Mouse monoclonal
SIGMA/SAB4200716 - clone VIM-13.2, purified from hybridoma cell culture
Synonym: Anti-F5H288
Product Type: Chemical
Enhanced Validation-By Independent Antibodies HS68 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 1.25 μg/mL Anti-Vimentin antibody, Mouse monoclonal, Clone: VIM-13.2 (Cat. No. SAB4200716) (A) and developed using Goat Anti-Mouse IgG, Cy3 conjugate, and 1 μg/mL Anti-Vimentin antibody, Mouse monoclonal, Clone: LN-6 (Cat. No. SAB4200761) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate antibody. Results Two Anti-Vimentin antibodies,SAB4200716 (A) and SAB4200761 (B) target different regions of Vimentin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence HS68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1.25 μg/mL Anti-Vimentin antibody, Mouse monoclonal, Clone: VIM-13.2 (Cat. No. SAB4200716).The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Anti-Vimentin antibody, Mouse monoclonal, Clone: VIM-13.2 (Cat. No. SAB4200716). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. C2C12; 2. HS68; 3.COS7; 4. BE; 5. 3T3; 6. MDCK; 7. MDBK; 8. CFB; 9. L8
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | VIM-13.2, monoclonal |
| concentration | ~1.0 mg/mL |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgM |
| mol wt | ~58 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, bovine, canine, rat, monkey, human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract |
| immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells | |
| immunohistochemistry: suitable |
| Application: | Anti-Vimentin antibody, Mouse monoclonal has been used in: • western blotting • indirect immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells. |
| Immunogen: | human foreskin fibroblast extract |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |