Anti-Laminin antibody, Mouse monoclonal
SIGMA/SAB4200719 - clone LAM-89, purified from hybridoma cell culture
Synonym: Anti-LAMA; Anti-LAMA1
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Formalin-fixed, paraffin-embedded Human Tongue sections stained with 1:30 Rabbit Anti-Laminin (Cat. No. L9393) (A). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate (Cat. No. F9887), and 40 μg/mL Anti-Laminin antibody, Mouse monoclonal, Clone: LAM-89 (Cat. No. SAB4200719) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Results Two Anti-Laminin antibodies L9393 (A) and SAB4200719 (B) target different regions of Laminin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human tongue sections with 40 μg/mL Anti-Laminin antibody, Mouse monoclonal, Clone: LAM-89 (Cat. No. SAB4200719). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Immunoblotting Laminin from murine sarcoma basement membrane (Cat. No.L2020) was separated on SDS-PAGE and probed with Anti-Laminin antibody, Mouse monoclonal, Clone: LAM-89 (Cat. No. SAB4200719). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.25 μg/mL antibody 4. 0.12 μg/mL antibody 5. 0.06 μg/mL antibody 6. 0.03 μg/mL antibody 7. Negative control: without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LAM-89, monoclonal |
| concentration | ~1.0 mg/mL |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~850 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | feline, human, porcine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| ELISA: suitable | |
| immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane | |
| immunofluorescence: suitable | |
| immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections. | |
| UniProt accession no. | P25391  |
| Application: | Monoclonal Anti-Laminin is recommended to use in various immunochemical assays, including immunohistochemistry, immunoblotting (~850 kDa), immunofluorescence, Enzyme linked immunosorbent assay (ELISA) and electron microscopy. |
| Biochem/physiol Actions: | Laminins contribute to the structure of the extracellular matrix (ECM). It also plays a major role in the regulation of variety of cellular processes, such as the rates of cell proliferation, differentiation, adhesion, migration, cell shaping, phenotype stability and resistance to apoptosis. Laminin polymers forms supramolecular network with collagen IV polymers through heparan sulfate proteoglycan and by nidogen (entactin) and plays an important role in the basement membrane (BM) stability. In mouse, mutations in the gene is associated with the development of muscular dystrophy. |
| Biochem/physiol Actions: | Monoclonal Anti-Laminin antibody specifically recognizes Laminin from human, feline and porcine origin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal anti-laminin antibody (mouse IgG1 isotype) is derived from the LAM-89 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified laminin from human placenta. Monoclonal anti-laminin antibody specifically recognizes laminin from human, feline and porcine origin. The antibody shows reactivity with laminin and specifically staining basal membrane of blood vessels, epithelium, nerve and muscle fiber. Monoclonal anti-laminin shows no reaction with collagen IV, fibronectin, vitronectin and chondroitin sulfate type A, B or C. Laminins are complexed extracellular glycoproteins which assemble the basal components of the basement membrane (BM). Each laminin is a heterotrimer (~850kDa) comprising of an α (~400kDa), β (~200kDa) and γ (~200kDa) chains all held together by disulfide bonds. |
| Immunogen: | purified Laminin from human Placenta |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |