Anti-β-Catenin antibody, Mouse monoclonal
SIGMA/SAB4200720 - clone 15B8, purified from hybridoma cell culture
Synonym: Anti-CTNNB1; Anti-armadillo
Product Type: Chemical
Immunofluorescence MCF7 cells were fixed and permeabilized with Methanol followed by Acetone. Fixed cells were stained with 10 μg/mL Anti-β Catenin,Mouse monoclonal, Clone: 15B8 (Cat. No. SAB4200720). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Anti-β Catenin, Mouse monoclonal, Clone: 15B8 (Cat. No. SAB4200720). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. 3T3L1; 3. L929; 4. MDBK; 5. COS7; 6. NRK; 7. BHK; 8. MDCK; 9. P19
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 15B8, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~94 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, mouse, human, rat, monkey, hamster, bovine, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract |
| immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P35222  |
| Application: | Anti-β-Catenin antibody, Mouse monoclonal has been used in: • immunoblotting • immunofluorescence • immunohistochemistry • immunoprecipitation |
| Biochem/physiol Actions: | β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily. |
| Immunogen: | recombinant chicken ß-Catenin |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |