Anti-GABA antibody, Mouse monoclonal
SIGMA/SAB4200721 - clone GB-69, purified from hybridoma cell culture
Synonym: Mouse Anti-
Product Type: Chemical
Immunohistochemistry
Staining of formalin-fixed, paraffin-embedded Human brain sections with 1 μg/mL Anti-GABA antibody, Mouse monoclonal, Clone: GB-69 (Cat. No. SAB4200721) using biotin/ExtrAvidin®-Peroxidase.
Staining of formalin-fixed, paraffin-embedded Human brain sections with 1 μg/mL Anti-GABA antibody, Mouse monoclonal, Clone: GB-69 (Cat. No. SAB4200721) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence AsPC-1 cells were fixed and permeabilized with 4% Paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Anti-GABA antibody, Mouse monoclonal, Clone: GB-69 (Cat. No. SAB4200721). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate (A). Cells were counterstained with DAPI (blue) to stain nuclei (B).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GB-69, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, monkey, frog, mouse, human, gerbil |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| ELISA: suitable | |
| immunofluorescence: 5-10 μg/mL using human pancreatic tumor AsPC1 cell line | |
| immunohistochemistry: 1-2.5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain and/or cerebellum sections |
| Application: | Anti-GABA antibody, Mouse monoclonal has been used in: • immunofluorescence • immunohistochemistry • enzyme linked immunosorbent assay (ELISA) • dot-blot |
| Biochem/physiol Actions: | γ-Aminobutyric acid (GABA) is the main inhibitory neurotransmitter. GABA mediates fast synaptic inhibition in the mature nervous system and plays multiple key roles as sensory circuits undergo functional development. GABA promotes proliferation of immune cells, embryonic stem cells and cortical progenitor cells. In addition, it is involved in protein synthesis and metabolism and plays a major role in the regulation of muscle tone, blood pressure, heart rate and respiration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-GABA antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GB-69 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified GABA conjugated to BSA. γ-Aminobutyric acid (GABA) is present in the central nervous system (CNS) of vertebrates. GABA is formed following enzymatic decarboxylation of |
| Immunogen: | purified GABA conjugated to BSA |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |