Anti-Band 3 antibody, Mouse monoclonal
SIGMA/SAB4200722 - clone BIII-136, purified from hybridoma cell culture
Synonym: AE 1; Anion exchange protein 1; Anion exchanger 1; Band 3 anion transport protein; Solute carrier family 4 member 1
Product Type: Chemical
Immunoblotting Total extract from human erythrocytes ghosts was separated on SDS-PAGE and probed with Anti-Band 3 antibody, Mouse monoclonal, Clone:BIII-136 (Cat. No. SAB4200722). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BIII-136, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 2-4 μg/mL (human erythrocytes ghosts extract) |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P02730   |
| Application: | Anti-Band 3 antibody, Mouse monoclonal may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | As a response to an oxidative stress, Band 3 protein forms clusters and clear damaged and aged RBCs from circulation. Enhanced band 3 protein clustering is implicated in RBC disorders such as hemolytic anemia, glucose-6-phosphate dehydrogenase (G6PD) deficiency, malaria and sickle-cell disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Band 3 antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the BIII-136 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with glycophorin B purified from human erythrocytes. |
| Physical form: | Glycophorin B purified from human erythrocytes |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |