Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal
SIGMA/SAB4200723 - clone WH-3, purified from hybridoma cell culture
Synonym: Anti-TPH; Anti-TpOH
Product Type: Chemical
Immunoblotting Total lysate from Rabbit pineal gland was separated on SDS-PAGE and probed with Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal, Clone: WH-3 (Cat. No. SAB4200723). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | WH-3, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG3 |
| mol wt | antigen ~55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rabbit, sheep, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 2-4 μg/mL using rabbit pineal gland lysate |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable |
| Application: | Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal has been used in: • immunoblotting • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | Tryptophan Hydroxylase (TPH) is a rate-limiting tetramer enzyme in the synthetic pathway of neurotransmitter serotonin from |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant rabbit Tryptophan Hydroxylase. Tryptophan Hydroxylase (TPH), also known as TpOH, TrpH or |
| Immunogen: | recombinant rabbit Tryptophan Hydroxylase. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |