Anti-Splicing Factor SC-35 antibody, Mouse monoclonal
SIGMA/SAB4200725 - clone SC-35, purified from hybridoma cell culture
Synonym: Anti-PR264; Anti-SFRS2; Anti-SFRS2A; Anti-SRp30b; Anti-Splicing component 35 kDa (SC35); Anti-
Product Type: Chemical
Immunofluorescence HeLa (A), COS-7 (B) and MDCK (C) cells were fixed and permeabilized with 4% Paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 0.25 μg/mL Anti-Splicing Factor SC-35 antibody, Mouse monoclonal, Clone: SC-35 (Cat. No. SAB4200725). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei and phalloidin to satin Actin (red).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SC-35, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~35 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, quail, hamster, frog, porcine, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| ELISA: suitable | |
| immunoblotting: suitable | |
| immunofluorescence: 0.125-0.25 μg/mL using HeLa cells | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | Q01130  |
| Application: | Anti-Splicing Factor SC-35 antibody has been used in • dot blot • enzyme linked immunosorbent assay (ELISA) • immunofluorescence • immunohistochemistry • immunoblotting • immunoprecipitation |
| Biochem/physiol Actions: | Splicing Factor SC-35 are critical regulators of constitutive and alternative pre-mRNA splicing. It also controls cellular apoptosis in response to genotoxic stress. Serine/arginine protein kinase 1 (SRPK1) specifically phosphorylates SC-35, resulting in p53 suppression and cyclin D1 upregulation. Elevated levels of SC-35 correlate with aggressive phenotype in several cancer types including lung squamous cell carcinoma and lung adenocarcinoma. SC-35 has oncogenic function in tumor progression of human papilloma virus (HPV)-infected cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Splicing Factor SC-35 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SC-35 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a RBF-DHJ mouse immunized with partially purified spliceosomes prepared from HeLa cells. Splicing Factor SC-35, also known as serine/arginine rich splicing factor 2 (SRSF2) belongs to the family of of serine/arginine-rich (SR) proteins. |
| Immunogen: | partially purified spliceosomes prepared from HeLa cells |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |