Anti-Connexin 43 antibody, Mouse monoclonal
SIGMA/SAB4200730 - clone CXN-6, hybridoma cell culture supernatant
Synonym: Anti-AVSD3; Anti-CMDR; Anti-CX43; Anti-EKVP; Anti-EKVP3; Anti-GJAL; Anti-HLHS1; Anti-HSS; Anti-ODDD; Anti-PPKCA
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded mouse heart sections with 1:200 Anti-Connexin 43 antibody, Mouse monoclonal, Clone: CXN-6 (Cat. No. SAB4200730) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence C2C12 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Connexin 43 antibody, Mouse monoclonal, Clone: CXN-6 (Cat. No. SAB4200730). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole tissue extracts were separated on SDS-PAGE and probed with 1:4000 Anti-Connexin 43 antibody, Mouse monoclonal, Clone: CXN-6 (Cat. No. SAB4200730). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Mouse brain 2. Rat brain
Western Blotting Whole C2C12 cells extract was separated on SDS-PAGE and probed with Anti-Connexin 43 antibody, Mouse monoclonal, Clone: CXN-6 (Cat. No. SAB4200730). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:2,500 2. Antibody dilution 1:5,000 3. Antibody dilution 1:7,500 4. Antibody dilution 1:10,000 5. Negative control: without first antibody
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CXN-6, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgM |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, rat, feline, bovine, human, porcine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1:5,000-1:7,500 using mouse myoblast C2C12 cell line extract |
| immunofluorescence: 1:500-1:1,000 using mouse myoblast C2C12 cell line | |
| immunohistochemistry: 1:200-1:500 using heat-retrieved formalin-fixed, paraffin-embedded mouse heart sections | |
| UniProt accession no. | P17302  |
| Biochem/physiol Actions: | Connexin 43 (Cx43) function is controlled by phosphorylation, SUMOylation and ubiquitination. The C-terminal tail of Cx43 modulates cytoskeletal dynamics and cell migration properties. Mutation in Cx43 leads to dysfunction of channel regulation domain and is associated with pathogenesis of hypoplastic left heart syndrome (HLHS). Cx43 dysregulation in testis contributes to infertility and testicular tumor. A gene mutation in the Cx43 results in abnormal development of eyes and teeth in oculodentodigital dysplasia (ODDD) , craniometaphyseal dysplasia and sudden infant death syndrome. Mutations in the GJA1 results in abnormal facial and skull development in hallermann-streiff syndrome (HSS). Missense mutations in GJA1 gene is also implicated in skin disorder called the, erythrokeratodermia variabilis et progressiva (EKVP) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Connexin 43 (Cx43) is also known as gap junction alpha-1 protein (GJA1). It is mapped to human chromosome 6q22.31. Cx43 is membrane protein with four transmembrane spanning regions, which comprise the gap junction channel. The loop connecting the transmembrane regions play a key role in channel docking. The N- and C-terminal amino acids are exposed to cytoplasm. |
| Immunogen: | synthetic peptide from the C-terminal region of Connexin 43 protein, conjugated to KLH |
| Physical form: | The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |