Anti-Pan-Cadherin antibody, Mouse monoclonal
SIGMA/SAB4200731 - clone CH-19, purified from hybridoma cell culture
Synonym: Anti-Pan Cadherin
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 μg/mL Anti-Pan-Cadherin antibody, Mouse monoclonal, Clone: CH-19 (Cat. No. SAB4200731). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 5 μg/mL Anti-Pan-Cadherin antibody, Mouse monoclonal, Clone: CH-19 (Cat. No. SAB4200731). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. NIH-3T3 3. COS7 4. MDBK 5. HEK-293
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CH-19, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~135 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | guinea pig, canine, frog, bovine, human, cat, monkey, rabbit, mouse, ferret, snake, hamster, chicken, Psammomys (sand rat), rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.125-0.25 μg/mL using HeLa cell extract |
| immunofluorescence: 5-10 μg/mL using HeLa cells | |
| immunohistochemistry: suitable | |
| UniProt accession no. | P12830  |
| P19022 |
|
| P22223 |
| Application: | Anti-Pan-Cadherin antibody, Mouse monoclonal may be used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-Pan-Cadherin recognizes Cadherin from human, mouse, monkey, bovine, chicken, rat, ferret, canine, rabbit, pig, guinea pig, hamster, cat, frog, snake and psammomys origin. Monoclonal Anti Pan-Cadherin is reactive against a highly conserved sequence at the cytoplasmic C-terminal of N-Cadherin protein. |
| Biochem/physiol Actions: | N-cadherin plays a key role in the neurulation, which involves transition of neural plate into neural tube. It is present in the synaptic membrane and dimerises upon synaptic induction and mediates synaptic activity. N-cadherin mediates cell−cell adhesion in retina but favors tumor progression in retinoblastoma. It is upregulated in carcinomas and is critical for cell invasion. Irregular distribution of N-cadherin is implicated in dilated cardiomyopathy. Mutations in the CHD2 gene may probably contribute to obsessive-compulsive disorder (OCD) and Tourette disorder (TD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Pan-Cadherin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CH-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with the synthetic peptide. |
| General description: | N-cadherin, also known as cadherin-2 (CDH2) or neural cadherin (NCAD) is mapped to human chromosome 18q12.1. N-cadherin has ectodomains comprising of 110 amino acidsand is called the extracellular cadherin (EC) domain. EC domains are critical for the adhesion function of N-cadherin. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of chicken N-Cadherin with an extra N-terminal lysine residue coupled with KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |