Anti-β-Tubulin I antibody, Mouse monoclonal
SIGMA/SAB4200732 - clone SAP.4G5, purified from hybridoma cell culture
Synonym: Anti-TUBB
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human fallopian tube sections with 2 μg/mL Anti-β-Tubulin I antibody, Mouse monoclonal, Clone: SAP.4G5 (Cat. No. SAB4200732) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human testis sections with 2 μg/mLAnti-β-Tubulin I antibody, Mouse monoclonal, Clone: SAP.4G5 (Cat. No. SAB4200732) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Anti-β-Tubulin I antibody, Mouse monoclonal, Clone: SAP.4G5 (Cat. No. SAB4200732). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 0.06 μg/mL Anti-β-Tubulin I antibody, Mouse monoclonal, Clone: SAP.4G5 (Cat. No. SAB4200732). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. Jurkat; 3. SHSY-5Y; 4. Rat brain; 5. PC-12; 6. NIH-3T3; 7. Mouse brain; 8. MDCK; 9. MDBK; 10. CHO; 11. COS7
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SAP.4G5, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract |
| immunofluorescence: 1-2.5 μg/mL using HeLa cells | |
| immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections. | |
| UniProt accession no. | P07437  |
| Application: | Anti-β-Tubulin I antibody, Mouse monoclonal has been used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | β-Tubulin I is also useful in deducing the role that different tubulin isotypes play in nerve development, function or maintenance. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-β-Tubulin I (mouse IgG1 isotype) is derived from the SAP.4G5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. Tubulin is the major building block of microtubules, an intracellular cylindrical filamentous structure, that is present in almost all eukaryotic cells. Tubulin exists in all cells as a 100 kDa protein, which assembles from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of 55 kDa and share considerable homology. |
| Immunogen: | synthetic peptide corresponding to the C-terminal sequence of ß-tubulin isotype I, conjugated to BSA |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |