Anti-IL6 high affinity antibody, Mouse monoclonal
SIGMA/SAB4200734 - clone IL6-2A3, purified from hybridoma cell culture
Synonym: Anti-B-cell stimulatory factor 2 (BSF-2); Anti-CTL differentiation factor (CDF); Anti-Hybridoma growth factor; Anti-Interferon beta-2 (IFN-beta-2); Anti-Interleukin-6
Product Type: Chemical
Immunoblotting Recombinant human IL6 was separated on SDS-PAGE and probed with Anti-IL6 high affinity antibody, Mouse monoclonal, Clone: IL6-2A3 (Cat. No. SAB4200734). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.06 μg/mL antibody 2. 0.125 μg/mL antibody 3. 0.25 μg/mL antibody 4. 0.5 μg/mL antibody 5. 0.5 μg/mL antibody + IL6 immunizing protein 6. 1 μg/mL antibody 7. Negative control: without first antibody
Immunoprecipitation Anti-IL6 high affinity antibody, Mouse monoclonal, Clone: IL6-2A3 (Cat. No. SAB4200734) was used to immunoprecipitate IL6 from Concentrated supernatant of IL-1b activated MG-63 cells. Detection antibody: Rabbit Anti-IL6, followed by Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1.10 μg/test antibody 2. 20 μg/test antibody 3. Negative control: 20 μg/test non-relevant antibody 4. 11 ng/lane Concentrated supernatant of IL-1b activated MG-63 cells
ELISA Anti-IL6 high affinity antibody, Mouse monoclonal, Clone: IL6-2A3 (Cat. No. SAB4200734) was serially diluted to detect recombinant human IL6 by indirect ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | IL6-2A3, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunoblotting: 0.125-0.25 μg/mL using recombinant human IL6 expressed in HEK-293T cells | |
| immunoprecipitation (IP): 10-20 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β | |
| UniProt accession no. | P05231  |
| Application: | Anti-IL6 high affinity antibody, Mouse monoclonal has been used in • immunoblotting • immunoprecipitation • ELISA |
| Biochem/physiol Actions: | Interleukin-6 (IL6) plays a wide variety of important biological roles in the immune, nervous and endocrine system. It is an essential component for hybridoma growth and is found in many supplemental cloning media. IL6 regulates acute and chronic inflammatory diseases and has been proven to be an excellent target for clinical treatment against autoimmune disorders such as rheumatoid arthritis, juvenile idiopathic arthritis and Castleman′s disease. IL6 also plays a key role in the pathogenesis of multiple myeloma. |
| General description: | Interleukin-6 (IL6) is a multifunctional cytokine. |
| Immunogen: | recombinant human IL6 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |