Anti-FITC antibody, Mouse monoclonal
SIGMA/SAB4200738 - clone FL-D6, purified from hybridoma cell culture
Synonym: Anti-Fluorescein isothiocyanate
Product Type: Chemical
Immunoblotting Extravidin-FITC (Cat. No. E2761) was separated on SDS-PAGE and probed with Anti-FITC antibody, Mouse monoclonal, Clone: FL-D6 (Cat. No. SAB4200738). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2.5 μg/mL antibody 2. 1.25 μg/mL antibody 3. 0.6 μg/mL antibody 4. 0.3 μg/mL antibody 5. 0.15 μg/mL antibody 6. 0.075 μg/mL antibody 7. Negative control: without first antibody
ELISA Anti-FITC antibody, Mouse monoclonal, Clone: FL-D6 (Cat. No. SAB4200738) was serially diluted to detect Extravidin-FITC (Cat. No. E2761) by indirect ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FL-D6, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC |
| immunohistochemistry: suitable | |
| indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC |
| Application: | Anti-FITC antibody, Mouse monoclonal may be used in: • immunoblotting • enzyme-linked immunosorbent assay (ELISA) • immunohistochemistry |
| Biochem/physiol Actions: | FITC (fluorescein isothiocyanate) is a commonly used protein marker including antibodies in immunofluorescent techniques. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten. |
| Biochem/physiol Actions: | Monoclonal Anti-FITC antibody specifically recognizes both free and conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate). |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-FITC antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FL-D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with FITC (fluorescein isothiocyanate)-BSA conjugate. This antibody specifically recognizes both free and conjugated FITC. FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm). |
| Immunogen: | FITC-BSA conjugate |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Discard working dilution samples if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |