Anti-Human IgE antibody, Mouse monoclonal
SIGMA/SAB4200741 - clone GE-1, purified from hybridoma cell culture
Synonym: Anti-Immunoglubulin E
Product Type: Chemical
Western Blotting Human IgE myeloma was separated on SDS-PAGE and probed with Anti-Human IgE antibody, Mouse monoclonal, Clone: GE-1 (Cat. No. SAB4200741). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 0.25 μg/mL antibody 3. 0.125 μg/mL antibody 4. 0.06 μg/mL antibody 5. 0.03 μg/mL antibody 6. Negative control: without first antibody
Immunoprecipitation Anti-Human IgE antibody, Mouse monoclonal, Clone: GE-1 (Cat. No. SAB4200741) was used to immunoprecipitate IgE from Human IgE myeloma. Detection antibody: Goat Anti-Human IgE (ε-chain specific)-Peroxidase (Cat. No. A9667) and a chemiluminescent substrate. Lanes 1.1 μg/test antibody 2. 2.5 μg/test antibody 3. 5 μg/test antibody 4. Negative control: 5 μg/test non-relevant antibody 5. Human IgE myeloma
ELISA Isotype specifity test: 0.15 μg/mL (blue) or 0.3 μg/mL (green) Anti-Human IgE antibody, Mouse monoclonal, Clone: GE-1 (Cat. No. SAB4200741) were used to detect Human IgE. Reactivity is observed only with human IgE subclass but not with human IgGs, IgA or IgM. Immunoglobulins from Mouse serum were used as negative control (purple).
ELISA Anti-Human IgE antibody, Mouse monoclonal, Clone: GE-1 (Cat. No. SAB4200741) was serially diluted to detect human IgE by indirect ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GE-1, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating |
| immunoblotting: suitable | |
| immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma |
| Application: | Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay. |
| Biochem/physiol Actions: | Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders. |
| General description: | Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml. |
| Immunogen: | purified human IgE myeloma protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |