Anti-c-Myc-Peroxidase antibody, Mouse monoclonal
SIGMA/SAB4200742 - clone 9E10, purified from hybridoma cell culture
Synonym: Anti-MRTL; Anti-Myc; Anti-bHLHe39; Anti-v-myc avian myelocytomatosis viral oncogene homolog
Product Type: Chemical
Western Blotting Whole extract of Human HEK-293T over-expressing c-Myc-MAPK1 cells was separated on SDS-PAGE and probed with Anti-c-Myc-Peroxidase antibody, Mouse monoclonal, Clone: 9E10 (Cat. No. SAB4200742). The antibody was developed using a chemiluminescent substrate. Lanes 1. 1:2,000 antibody dilution 2. 1:4,000 antibody dilution
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9E10, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG1 |
| packaging | vial of 100 μL |
| vial of 500 μL | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1:250-1:500 using lysate of HEK-293T cells over expressing c-Myc fusion protein |
| immunohistochemistry: suitable | |
| UniProt accession no. | P01106  |
| Application: | Anti-c-Myc-Peroxidase antibody, Mouse monoclonal may be used in immunoblotting and immunohistology. |
| Biochem/physiol Actions: | c-Myc is a transcription factor involved in regulation of cell growth, proliferation, differentiation and apoptosis. It is implicated in DNA replication, RNA splicing or transcription. Increased expression of the gene is associated with the development of various types of human cancers. |
| Biochem/physiol Actions: | Monoclonal Anti-Calponin specifically recognizes smooth muscle Calponin. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide of the human p62c-myc protein, conjugated to KLH. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain. The human c-Myc proto-oncogene is the cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses. |
| Immunogen: | Synthetic peptide of the human p62c-myc protein. |
| Physical form: | Supplied as lyophilized powder. After reconstitution with 0.1 mL/0.5 ml of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline |
| Preparation Note: | Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |