Anti-BDNF antibody, Mouse Monoclonal
SIGMA/SAB4200744 - clone BDNF129-13, purified from hybridoma cell culture
Synonym: Anti-Abrineurin; Anti-Brain-derived neurotrophic factor
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Cerebellum sections with 20 μg/mL Anti-BDNF antibody, Mouse monoclonal, Clone: BDNF129-13 (Cat. No. SAB4200744) using Biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Cerebellum sections with 20 μg/mL Anti-BDNF, mouse antibody, Clone: BDNF129-13 (Cat. No. SAB4200744) using Biotin/ExtrAvidin®-Peroxidase
Western Blotting Cell lysates were separated on SDS-PAGE and and probed with 4 μg/mL Anti-BDNF antibody, Mouse monoclonal, Clone: BDNF129-13 (Cat. No. SAB4200744). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. BDNF recombinant protein (Cat.No. B3795) 2. Supernatant from Human HEK-293T over-expressing BDNF 3. Human HEK-293T over-expressing BDNF
Immunoprecipitation Anti-BDNF antibody, Mouse monoclonal, Clone BDNF129-13 (Cat. No. SAB4200744) was used to immunoprecipitate BDNF from Human HEK-293T over-expressing BDNF cell lysate. Detection antibody: Rabbit Anti-Flag (Cat. No. F7425) , followed by Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Negative control: without lysate 2. Negative control: without antibody 3. 10 μg/test antibody 4. 20 μg/test antibody 5. 40 μg/test antibody 6. Positive control: Human HEK-293T over-expressing BDNF total lysate
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BDNF129-13, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | ~12 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein |
| immunocytochemistry: suitable | |
| immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections | |
| immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein | |
| UniProt accession no. | P23560  |
| Application: | Anti-BDNF antibody, Mouse Monoclonal has been used in: • immunoblotting • immunohistochemistry • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis through the specific receptor tropomyosin-related kinase B (TrkB). Changes in BDNF expression have been extensively investigated in depression, drug addition, schizophrenia, bipolar and anxiety disorders, Huntington′s disease and Rett syndrome. In addition, BDNF is associated with Alzheimer′s disease (AD) and its reduced levels were detected in post-mortem brain tissues of AD patients. It can exert profound effects on development, morphology, synaptic plasticity and brain functions. |
| General description: | Anti-BDNF antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the BDNF129-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Brain-derived neurotrophic factor (BDNF), also known as Abrineurin, is a secreted dimeric protein, which belongs to the neurotrophin family of growth factors, along with nerve growth factor (NGF) and neurotrophins 3, 4/5 and 6. BDNF is widely expressed in the central nerve system (CNS). |
| Immunogen: | Synthetic peptide corresponding to a region located in the mature human BDNF protein conjugated to KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |