Anti-Protein A antibody, Mouse Monoclonal
SIGMA/SAB4200745 - clone SPA-27, purified from hybridoma cell culture
Synonym: Anti-IgG binding protein A; Anti-SpA; Anti-Staphylococcal protein A; Anti-Staphylococcus ayreus protein A
Product Type: Chemical
Western Blotting Protein A from Staphylococcus aureus (Cat. No. P6031) was separated on SDS-PAGE and probed with Anti-Protein A antibody, Mouse monoclonal, Clone: SPA-27 (Cat. No. SAB4200745). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.25 μg/mL antibody 4. 0.125 μg/mL antibody 5. Negative control: without first antibody
Immunoprecipitation Anti-Protein A antibody, Mouse monoclonal, Clone: SPA-27 (Cat. No. SAB4200745) was used to immunoprecipitate Protein A from Staphylococcus aureus (Cat. No. P6031). Detection antibody: Rabbit Anti-Protein A (Cat. No. P3775), followed by Monoclonal Anti-Rabbit IgG-Peroxidase (Cat. No. R3155) and a chemiluminescent substrate. Lanes 1. 1 μg/test antibody 2. 2.5 μg/test antibody 3. 5 μg/test antibody 4. Negative control: 5 μg/test without lysate 5. Negative control: 5 μg/test non-relevant antibody 6. Negative control: without antibody 7. Protein A from Staphylococcus aureus
ELISA Anti-Protein A antibody, Mouse monoclonal, Clone: SPA-27 (Cat. No. SAB4200745) was serially diluted to detect Protein A from Staphylococcus aureus (Cat. No. P6031) by indirect ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SPA-27, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Staphylococcus aureus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| flow cytometry: suitable | |
| immunoblotting: suitable | |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): 2.5-5 μg/test using purified protein A from Staphylococcus aureus | |
| indirect ELISA: 0.25-0.5 μg/mL using 10 μg/mL of protein A from Staphylococcus aureus for coating. To reduce nonspecific binding we recommend on detection using a secondary anti-mouse, F(ab’)2 fragment antibody |
| Application: | The antibody may be used in various immunochemical techniques including immunoblotting, dot blot, ELISA, immunofluorescence, immunoprecipitation and flow cytometry (FACS). |
| Biochem/physiol Actions: | Monoclonal Anti-Protein A specifically recognizes Protein A from Staphylococcus aureus bacteria. |
| Biochem/physiol Actions: | Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used either in its native form, conjugated to various markers or serve as a tag for a specific protein of interest. In addition, protein A has low affinity to mouse monoclonal antibodies from IgG1 isotype. Thus, a monoclonal anti-protein A antibody of the mouse IgG1 isotype has an advantage due to its specific targeting and binding to protein A via its Fab fragment. Monoclonal anti-protein A antibody can serve a useful tool for quantification and visualization of S. aureus bacteria and bacterial contaminations in different examined samples and for detection of protein A tagged proteins. |
| Disclaimer: | This product is for R&D use only, not for drug, household, or other uses. Please consult the material safety data sheet (MSDS) for information regarding hazards and safe handling practices. |
| General description: | Staphylococcus aureus protein A, also known as SpA or IgG binding protein A, is a 42 kDa single chain polypeptide isolated from the cell wall of S. aureus Cowan I strain. It consists of a single polypeptide chain containing five repetitive immunoglobulin-binding domains, rich in aspartic and glutamic acids.Anti-Protein A antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SPA-27 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse. |
| Immunogen: | Protein A from Staphylococcus aureus Cowan I strain |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |