Anti-Neurofilament 200 antibody, Mouse monoclonal
SIGMA/SAB4200747 - clone NE14, purified from hybridoma cell culture
Synonym: Anti-H-subunit; Anti-NF-H
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Rat Cerebellum sections with 5 μg/mL Anti-Neurofilament 200 antibody, Mouse monoclonal, Clone: NE14 (Cat. No. SAB4200747) using FITC-Anti-Mouse IgG (Cat. No. F5262)
Western Blotting Rat brain S1 fraction extract were separated on SDS-PAGE and probed with Anti-Neurofilament 200 antibody, Mouse monoclonal, Clone: NE14 (Cat. No. SAB4200747). The antibody was developed using 1:3,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. 0.5 μg/mL antibody 5. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NE14, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~200 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | feline, rat, guinea pig, pig, human, mouse, bovine, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1.25-2.5 μg/mL using rat brain S1 fraction |
| immunohistochemistry: 5-10 μg/mL using enzyme treated formalin-fixed, paraffin-embedded rat brain or mouse brain sections | |
| UniProt accession no. | P12036  |
| Application: | Anti-Neurofilament 200 antibody, Mouse monoclonal has been used in immunoblotting and immunohistochemistry staining. |
| Biochem/physiol Actions: | Neurofilaments are the type of intermediate filaments (IFs), that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. It has an important function in mature axons that is not sub served by the two smaller neurofilament proteins. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion. Neurofilaments can accumulate in large numbers within cell bodies and proximal axons of affected neurons in several pathological diseases, such as Charcot-Marie-Tooth (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, neurofilament accumulations was detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients. |
| General description: | Anti-Neurofilament 200 Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NE14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as neurofilament heavy polypeptide (Hsubunit), NF-H, NEFH or 200 kDa neurofilament protein. |
| Immunogen: | Neurofilaments purified from pig spinal cord |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |