Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse
SIGMA/SAB4200748 - clone MB-11, purified immunoglobulin
Synonym: Anti-Human immunoglobulin M
Product Type: Chemical
ELISA Monoclonal anti-Human IgM-Peroxidase antibody, Clone: MB-11 (Cat. No. SAB4200748) was serially diluted to detect human IgM by direct ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MB-11, monoclonal |
| concentration | ~2.0 mg/mL |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40,000-1:80,000 using 2.5 μg/mL Human IgM for coating |
| Application: | Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse may suitable for use in enzyme-linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | IgM is suggested to act as the first line of defense during microbial infections. Secreted IgM (sIgM) is involved in B cell maturation and complement activation. Detection of IgM antibodies in a patient′s serum may indicates a recent infection and IgM in a neonate′s serum may indicate on intrauterine infection (e.g. congenital rubella syndrome). In addition, the development of anti-donor IgM after combined liver-kidney transplantation has been shown to provide a graft- protecting effect. Anti-Human IgM (μ-specific) antibodies can be used in detection of the abnormal concentration of IgM in patient sera and IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMD) and the hyperimmunoglobulin M (hyper-IgM or HIGM) syndromes. |
| Biochem/physiol Actions: | Monoclonal Anti-Human IgM (μ-chain) specifically recognizes μ chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, kappa, lambda and gamma chains. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgM protein. Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocytes membranes. IgM has a pentameric structure, in which monomers are linked together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells. |
| Immunogen: | purified human IgM protein |
| Physical form: | Supplied as a lyophilized powder |
| Preparation Note: | Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |