Anti-Collagen Type III antibody, Mouse monoclonal
SIGMA/SAB4200749 - clone FH-7A, purified from hybridoma cell culture
Synonym: Anti-COL3A1; Anti-Collagen alpha-1(III) chain
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat skin sections with 10 μg/mL Anti-Collagen III antibody, Mouse monoclonal, Clone: FH-7A (Cat. No. SAB4200749). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FH-7A, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunoblotting: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat skin sections. | |
| UniProt accession no. | P02461  |
| Biochem/physiol Actions: | Collagen a-1(III) (COL3A1) serves as a potent adjunct marker for both differentiating fibroadenoma (FA) from phyllodes tumor (PT) and measuring malignant potential in PTs. Overexpression of the gene in glomerular leads to the development of collagen type III glomerulopathy. Mutations in the gene is associated with the development of familial aortic aneurysms and Ehlers-Danlos syndrome. |
| General description: | Anti-collagen type III antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse. Monoclonal anti-collagen type III specifically recognizes native and denatured collagen type III from human 1 and rat 2 origin. It does not recognize collagen types I, II, IV, V, VI and X. Collagen type III, also known as collagen α-1(III) (COL3A1), is encoded by the gene mapped to human chromosome 2q24.3-q31. It is the highly expressed collagen in blood vessels and hollow organs. |
| Immunogen: | Human Collagen Type III |
| Other Notes: | In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |