Anti-Gelsolin antibody, Mouse Monoclonal
SIGMA/SAB4200750 - clone GS-2C4, purified from hybridoma cell culture
Synonym: Anit-AGEL; Anit-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human pancreas sections with 10 μg/mL Anti-Gelsolin antibody, Mouse monoclonal, Clone: GS-2C4 (Cat. No. SAB4200750) using biotin/ExtrAvidin®-Peroxidase.
Western Blotting Whole extract of COS-7 cells was separated on SDS-PAGE and probed with Anti-Gelsolin antibody, Mouse monoclonal, Clone: GS-2C4 (Cat. No. SAB4200750). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2.5 μg/mL antibody 3. 5 μg/mL antibody 4. Negative control: without first antibody
Western Blotting Whole extract of MDCK cells was separated on SDS-PAGE and probed with Anti-Gelsolin antibody, Mouse monoclonal, Clone: GS-2C4 (Cat. No. SAB4200750). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2.5 μg/mL antibody 3. 5 μg/mL antibody 4. Negative control: without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GS-2C4, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~85 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, canine, human, mouse, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1-2.5 μg/mL using MDCK cell line extract |
| immunofluorescence: suitable | |
| immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P06396  |
| Application: | Anti-Gelsolin antibody, Mouse Monoclonal has been used in: • immunoblotting • immunofluorescence • immunohistochemistry • immunoprecipitation |
| Biochem/physiol Actions: | Gelsolin, also known as Actin-depolymerizing factor (ADF), GSN, AGEL or Brevin, is a calcium-dependent actin-binding protein. Gelsolin acts as a main modulator of actin filament formation and disassembly and is involved in a variety of cellular mechanisms including apoptosis, signal transduction, transcriptional regulation and epigenetic processes. Plasma gelsolin differs in its sequence from the cytoplasmic variant in possessing a 25-amino acid N-terminal extension. The main function of the cytoplasmic gelsolin is to remodel the actin cytoskeleton. Plasma gelsolin′s main role is to scan for actin fibrils that are released by injured tissue into the bloodstream and to bind them in a scavenger mode preventing actin from increasing the blood viscosity. Gelsolin levels correlate with inflammation and several diseases and pathologies, including cancer, infection, cardiac injury, pulmonary diseases, Alzheimer′s disease and aging thus making it a potential biomarker of diagnostic and prognostic values. |
| General description: | Anti-Gelsolin Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GS-2C4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Gelsolin operates in at least two variant forms encoded by a single gene and is located both intracellular (in cytosol and mitochondria) and extracellular (in blood plasma). |
| Immunogen: | Human plasma gelsolin |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |