Anti-PLA2R antibody, Mouse monoclonal
SIGMA/SAB4200757 - clone 12-6-5, purified from hybridoma cell culture
Synonym: Anti-180 kDa secretory phospholipase A2 receptor; Anti-C-type lectin domain family 13 member C; Anti-M-type receptor; Anti-PLA2-R; Anti-PLA2R; Anti-Secretory phospholipase A2 receptor
Product Type: Chemical
Western Blotting Extract from PLA2R recombinant protein was separated on SDS-PAGE and probed with Anti-PLA2R antibody, Mouse monoclonal, Clone:12-6-5 (Cat. No. SAB4200757). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 2 μg/mL Mouse Anti-PLA2R antibody 2. 1 μg/mL Mouse Anti-PLA2R antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 12-6-5, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | ~180 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunoblotting: 1-2 μg/mL using partial recombinant human PLA2R1 protein (predicted ~150kDa) | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| UniProt accession no. | Q13018  |
| Application: | Anti-PLA2R antibody has been used in • immunoblotting • immunofluorescence • flow Cytometry • immunohistochemistry |
| Biochem/physiol Actions: | Secretory phospholipase A2 receptor (PLA2R) acts as a receptor for secretory phosholipase A2 (sPLA2) allowing its removal from circulation, thus regulating its biological effect. PLA2R is also involved in clearance followed by suppression of their potent enzymatic activities of secretory phospholipase A2 (sPLA2) from the blood, including group sPLA2-X and a snake venom sPLA2. The soluble circulating form of PLA2R is constitutively present as an endogenous inhibitor of sPLA2s. PLA2R is expressed in kidney podocytes of normal human glomeruli, in idiopathic membranous nephropathy (IMN) patients PLA2R is colocalized to immune deposits in glomeruli along with IgG4. |
| General description: | Anti-PLA2R antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the 12-6-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with recombinant fragment of human PLA2R corresponding to extracellular sequence NC3. Secretory phospholipase A2 receptor (PLA2R) is 180 kDa and is a type I transmembrane glycoprotein member of the mannose receptor (MR) family. Large extracellular glycosylated region comprising an N-terminal cysteine-rich domain (CysR), a fibronectin-like type II domain (FnII), and eight to ten C-type lectin-like domains (CTLD). |
| Immunogen: | Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CT |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |