Anti-Human IgG3 antibody, Mouse monoclonal
SIGMA/SAB4200759 - clone HP-6050, purified from hybridoma cell culture
Synonym: Anti-Immunoglobulin G3
Product Type: Chemical
Immunoprecipitation Anti-Human IgG3 antibody, Mouse monoclonal,Clone: HP-6050 (Cat. No. SAB4200759) was used to immunoprecipitate Human IgG3 myeloma protein (Cat. No. I5654). Detection antibody: Goat Anti-Human IgG (Fc specific)-Peroxidase (Cat. No. A0170) and a chemiluminescent substrate. Lanes 1. 1 μg/test antibody 2. 2.5 μg/test antibody 3. 5 μg/test antibody 4. Negative control: 5 μg/test without lysate 5. Negative control: 5 μg/test non-relevant antibody 6. Negative control: without antibody 7. Human IgG3 myeloma protein
ELISA Anti-Human IgG3 antibody, Mouse monoclonal Clone: HP-6050 (Cat. No. SAB4200759) was serially diluted to detect Human IgG3 (Cat. No. I5654) by indirect ELISA.
ELISA Isotype specifity test: 0.15 μg/mL (blue) or 0.3 μg/mL (red) Anti-Human IgG3 antibody, Mouse monoclonal, Clone: HP-6050 (Cat. No. SAB4200759) were used to detect Human IgG3 (Cat. No. I5654). Reactivity is observed only with human IgG3 subclass (both κ and λ light chains) but not with human IgG1, IgG2 or IgG4.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HP-6050, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1-2.5 μg/test using Human IgG3 myeloma |
| indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml Human IgG3 myeloma for coating |
| Application: | Anti-Human IgG3 antibody, mouse monoclonal has been used in indirect ELISA and immunoprecipitation. |
| Biochem/physiol Actions: | IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial . IgG3 deficiency has been associated with a history of recurrent infectious, leading to chronic lung disease. |
| General description: | Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. The abundance of the different IgG subclasses in the bloodstream varies with age, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels rise more slowly, reaching adult levels at about 10 years of age. |
| Immunogen: | Human IgG3 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |