Anti-Fibronectin antibody, Mouse monoclonal
SIGMA/SAB4200760 - clone FN-15, purified from hybridoma cell culture
Synonym: Anti-CIG; Anti-Cold-insoluble globulin; Anti-FN
Product Type: Chemical
Immunofluorescence Hs68 cells were fixed and permeabilized with methanol followed by aceton. Fixed cells were stained with 2.5 μg/mL Anti-Fibronectin antibody, Mouse monoclonal, Clone: FN-15 (Cat. No. SAB4200760). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of human WI-38 cells was separated on SDS-PAGE and probed with Anti-Fibronectin antibody, Mouse monoclonal, Clone: FN-15 (Cat. No. SAB4200760). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1.25 μg/mL antibody 2. 0.625 μg/mL antibody 3. 0.31 μg/mL antibody 4. 0.15 μg/mL antibody 5. 0.07 μg/mL antibody 6. 0.03 μg/mL antibody 7. Positive control 1:1000 mouse Anti-Fibronectin, Clone: FN-15 ascites fluid (Cat. No. F7387) 8. Negative control: without first antibody
ELISA Anti-Fibronectin antibody, Mouse monoclonal, Clone: FN-15 (Cat. No. SAB4200760) was serially diluted to detect Fibronectin from Human plasma (Cat. No. F2006) by indirect ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FN-15, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 220-260 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.3-0.6 μg/mL using human fetal fibroblast-like WI-38 cell line extract |
| immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating | |
| UniProt accession no. | P02751  |
| Application: | The antibody may be used in various immunochemical techniques including Immunoblotting (∼220-260kDa)1, ELISA, Immunofluorescence, Immunohistochemistry and Immunoprecipitation. |
| Biochem/physiol Actions: | Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human and mouse origin. |
| Biochem/physiol Actions: | The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue. |
| General description: | Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. Fibronectin (FN), also known as Cold-insoluble globulin (CIG), is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN1 gene 6. In vertebrates, two types of Fibronectin are present: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue. |
| Immunogen: | Fibronectin isolated from human plasma. |
| Other Notes: | In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test. |
| Physical form: | Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |