Anti-Dystrophin antibody, Mouse monoclonal
SIGMA/SAB4200763 - clone MANDRA1, purified from hybridoma cell culture
Synonym: Anti-DMD
Product Type: Chemical
Immunohistochemistry
Staining of Frozen, Acetone-fixed, Rat tongue sections with 20 μg/mL Anti-Dystrophin antibody, Mouse monoclonal, Clone: MANDRA1 (Cat. No. SAB4200763). The antibody was developed using Goat Anti-Mouse IgG FITC conjugate.
Staining of Frozen, Acetone-fixed, Rat tongue sections with 20 μg/mL Anti-Dystrophin antibody, Mouse monoclonal, Clone: MANDRA1 (Cat. No. SAB4200763). The antibody was developed using Goat Anti-Mouse IgG FITC conjugate.
Enhanced Validation-By Independent Antibodies Frozen, Acetone fixed Rat tounge sections were stained with 20 μg/mL Anti-Dystrophin antibody, Mouse monoclonal, Clone: MANDRA1 (Cat. No. SAB4200763) (A) and developed using Goat Anti-Mouse IgG, FITC conjugate, and 20 μg/mL Anti-Dystrophin antibody, Mouse monoclonal, Clone: MANDYS8 (Cat. No. SAB4200764) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate antibody. Results Two Anti-Dystrophin antibodies,SAB4200763 (A) and SAB4200764 (B) target different regions of Dystrophin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MANDRA1, monoclonal |
| concentration | ~1.0 mg/mL |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~427 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | zebrafish, rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: 10-20 μg/mL using acetone fixed rat tongue frozen sections | |
| UniProt accession no. | P11532  |
| Application: | Anti-Dystrophin antibody, Mouse monoclonal has been used in: • immunohistochemistry • immunoblotting • immunofluorescence • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Anti-Dystrophin antibody, Mouse monoclonal specifically recognizes an epitope located on the 128 amino acids at the end of the C-terminal domain of the human Dystrophin molecule (residues 3558-3684). The antibody reacts with Dystrophin from human, mouse, rat and zebrafish origin. The antibody is specific to dystrophin and does not react with -actinin or utrophin. |
| Biochem/physiol Actions: | Dystrophin is absent, reduced or altered because of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or in its homologue in mouse. Severe DMD is associated with a marked Dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Dystrophin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the MANDRA1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dystrophin is a rod-shaped cytoskeletal protein located to the periphery (plasma membrane) of normal striated muscle fibers. |
| General description: | Dystrophin is encoded by the gene mapped to human chromosome Xp21.2-p21.1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |