Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal
SIGMA/SAB4200766 - clone NOS-IN, purified from hybridoma cell culture
Synonym: Anti-Hepatocyte NOS (HEP-NOS); Anti-Inducible NOS (iNOS); Anti-NOS type II; Anti-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human liver sections with 20 μg/mL Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal, Clone: NOS-IN (Cat. No. SAB4200766) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human pancreas sections with 20 μg/mL Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal, Clone: NOS-IN (Cat. No. SAB4200766) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence RAW 264.7 cells activated with lipopolysaccharide (LPS) and interferon-γ, were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 10 μg/mL Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal, Clone: NOS-IN (Cat. No. SAB4200766). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of RAW 264.7 cells activated with lipopolysaccharide (LPS) and interferon-γ was separated on SDS-PAGE and probed with Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal, Clone: NOS-IN (Cat. No. SAB4200766). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 2 μg/mL antibody 4. 4 μg/mL antibody 5. Negative control: without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NOS-IN, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, plant |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunoblotting: 2-4 μg/mL using mouse macrophage RAW 264.7 cell line activated with lipopolysaccharide (LPS) and interferon-γ | |
| immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections | |
| UniProt accession no. | P35228  |
| Application: | Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal may be used: • immunoblotting • immunofluorescence • immunohistochemistry • fluorescence-activated cell sorting (FACS) |
| Biochem/physiol Actions: | Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal reacts specifically with inducible-type nitric oxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain (bNOS) or with endothelial-type NOS (eNOS). The antibody recognizes iNOS from human, mouse, rat and plant origin. |
| Biochem/physiol Actions: | Inducible nitric oxide synthase (iNOS) or NOS2 catalyzes the oxidation of |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Inducible-type nitric oxide synthase (NOS) of mouse macrophage origin (iNOS) or macrophage NOS (mNOS) is a type II NOS (NOS2). It is a Ca2+-independent protein, expressed in activated macrophages and some glial cells after stimulation. NOS2 is also present in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts. The iNOS gene is mapped to human chromosome 17q11.2. iNOS is a dimeric flavoprotein with a heme group. It comprises the C-terminal reductase domain and an N-terminal oxygenase domain. |
| Immunogen: | synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |