Anti-Cathepsin D antibody, Mouse monoclonal
SIGMA/SAB4200767 - clone CTD-19, purified from hybridoma cell culture
Synonym: Anti-CTSD, cleaved into the following 2 chains: Cathepsin D light chain and Cathepsin D heavy chain
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human liver sections with10 μg/mL Anti-Cathepsin D antibody, Mouse monoclonal, Clone: CTD-19 (Cat. No. SAB4200767) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human breast cancer sections with 5 μg/mL Anti-Cathepsin D antibody, Mouse monoclonal, Clone: CTD-19 (Cat. No. SAB4200767) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with10 μg/mL Anti-Cathepsin D antibody, Mouse monoclonal, Clone: CTD-19 (Cat. No. SAB4200767). The antibody was developed using Goat Anti-Mouse IgG, ATTO488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Anti-Cathepsin D antibody, Mouse monoclonal, Clone: CTD-19 (Cat. No. SAB4200767). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HT-29; 2. MCF-7; 3. PC3; 4. NIH-3T3; 5. COS7; 6. CHO; 7. MDBK; 8. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CTD-19, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | human, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 2-4 μg/mL using human breast cancer MCF7 cell line |
| immunofluorescence: 5-10 μg/mL using HeLa cells | |
| immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections | |
| UniProt accession no. | P07339  |
| Application: | Anti-Cathepsin D antibody, Mouse monoclonal has been used in: • immunoblotting • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | CatD plays numerous physiological functions in the cells including metabolic degradation of intracellular proteins and the activation of enzymatic precursors. In the central nervous system, CatD is particularly important for the control of neuronal homeostasis, cell migration and interneuron communication. CatD-mediated proteolysis mediates the degradation of unfolded/oxidized protein aggregates in lysosome. The level of CatD synthesized by the cells is increased in response to mitogenic signals from estrogen, epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin like growth factor-I (IGF- I). The ability of tumor cells to invade the extracellular matrix has been attributed to cathepsins released by tumor cells or associated with its plasma membrane. |
| General description: | Anti-Cathepsin D antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the CTD-19 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with cathepsin D purified from human liver. Cathepsin D (CatD or CTSD) is synthesized in the rough endoplasmic reticulum as pre-proprotein. After removal of signal peptide, the pro-CatD is targeted to endosomes to form an active, ~48 kDa, single-chain intermediate then to the lysosomes to form the fully active mature protease, composed of a ~30 kDa heavy chain and a ~14 kDa light chain. |
| Immunogen: | Cathepsin D Purified from human liver |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |