Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal
SIGMA/SAB4200768 - clone 8c/6-39, purified from hybridoma cell culture
Synonym: Anti-Human immunoglobulin G1
Product Type: Chemical
ELISA Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal, Clone: 8c/6-39 (Cat. No. SAB4200768) was serially diluted to detect human IgG1 by direct ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8c/6-39, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | peroxidase conjugate |
| form | lyophilized |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating |
| Application: | The antibody is recommended to use in various immunological techniques, including ELISA. |
| Biochem/physiol Actions: | The immunoglobin G (IgG) subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the main antibody produced against tetanus toxoid antibodies. Elevation of IgG1 levels has been found in the cerebral spinal fluid of patients with multiple sclerosis. |
| General description: | Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.Monoclonal Anti-Human IgG1 specifically recognizes Human IgG1. The antibody shows no cross-reactivity with human IgG2, IgG3, or IgG4. This clone has been established as a useful human IgG1 specificity standard by the WHO/IUIS. |
| General description: | Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the 8c/6-39 hybridoma (also known as HP6019), produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the Fc fragment of a human IgG1 myeloma protein. |
| Immunogen: | The Fc fragment of a human IgG1 myeloma protein |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. |
| Physical form: | Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |