Anti-Human IgG3-Peroxidase antibody, Mouse monoclonal
SIGMA/SAB4200769 - clone HP-6050, purified from hybridoma cell culture
Synonym: Anti-Human immunoglobulin G3
Product Type: Chemical
ELISA Anti-Human IgG3-Peroxidase antibody, Mouse monoclonal, Clone: HP-6050 (Cat. No. SAB4200769) was serially diluted to detect human IgG3 by direct ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HP-6050, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:80,000-1:160,000 using 2 μg/mL human IgG3 for coating. |
| Application: | Direct ELISA: a working dilution of 1:80,000-1:160,000 is recommended using 2 μg/mL human IgG3 for coating. |
| General description: | Human IgG consist of four subclasses (1-4) that can be recognized by the antigenic difference in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens responses give rise to IgG1 and IgG3 antibodies. IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial cells, while IgG2 and IgG4 are far less efficient. The abundance of the different IgG subclasses in the bloodstream varies with age, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels rise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. IgG3 deficiency has been associated with a patient’s history of recurrent infectious that may lead to chronic lung disease. Decreased IgG3 levels are frequently associated with IgG1 deficiency. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the related immunological processes and may assist in the diagnosis of various disorders. |
| Immunogen: | Human IgG3 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads |
| Other Notes: | In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test. |
| Physical form: | Supplied as a lyophilized powder. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |