Anti-Pentraxin 3 antibody, Mouse monoclonal
SIGMA/SAB4200773 - clone PTX3-5, purified from hybridoma cell culture
Synonym: Pentaxin-related protein PTX3; TNF alpha-induced protein 5; TSG-14; Tumor necrosis factor alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein
Product Type: Chemical
Western Blotting Whole extract of Human HEK-293T over-expressing Pentraxin 3 was separated on SDS-PAGE and probed with Anti-Pentraxin 3 antibody, Mouse monoclonal, Clone: PTX3-5 (Cat. No. SAB4200773). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + Pentraxin 3 recombinant protein 3. Negative control: without first Antibody
Western Blotting Pentraxin 3 recombinant protein (Cat. No. MSST0004) was separated on SDS-PAGE and probed with Anti-Pentraxin 3 antibody, Mouse monoclonal, Clone: PTX3-5 (Cat. No. SAB4200773). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 0.25 μg/mL antibody 3. Negative control: without first Antibody
Immunoprecipitation Anti Pentraxin 3 antibody, Mouse monoclonal, Clone: PTX3-5 (Cat. No. SAB4200773) was used to immunoprecipitate Pentraxin 3 from Human HEK-293T over-expressing Pentraxin 3 cells lysate. Detection antibody: Rabbit Anti-Pentraxin 3 followed by Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/test antibody 2. 2 μg/test antibody 3. 4 μg/test antibody 4. Negative control: 5 μg/test non-relevant antibody 5. Negative control: without antibody 6. Negative control: without cell lysate 7. Human HEK-293T over-expressing Pentraxin 3 cells lysate
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PTX3-5, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~47 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.5-1 μg/mL using purified recombinant human Pentraxin 3 protein |
| immunoprecipitation (IP): 1-2 μg/test using lysate of HEK-293T cells over-expressing human Pentraxin 3 | |
| UniProt accession no. | P26022  |
| Application: | Anti-Pentraxin 3 antibody, Mouse monoclonal may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-Pentraxin 3 antibody, Mouse monoclonal recognizes both glycosylated and non-glycosylated human PTX3. |
| Biochem/physiol Actions: | Pentraxin 3 (PTX3) is released in response to primary inflammatory signals, such as toll-like receptor engagement, tumor necrosis factor α (TNFα) and interleukin 1β (IL-1β). It regulates innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. PTX3 acts as a soluble pathogen recognition receptor and aids resistance against pathogens. It participates in the removal of apoptotic cells during an immune response. PTX3 associates with complement factor H and associates with synovial fluid in rheumatoid arthritis and also in conditions of sepsis. PTX3 is suppressed by pitavastatin, making it a biomarker for inflammatory cardiovascular disease. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Pentraxin 3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the PTX3-5 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with recombinant human pentraxin 3 protein expressed in HEK-293 cells. Pentraxin 3 (PTX3), also called tumor necrosis factor inducible gene 14 protein (TSG-14), is a highly glycosylated secreted protein usually found as covalently-bound homooctamers. PTX3 belongs to the pentraxin subfamily, sharing the C-terminal domain with short pentraxins and contains a unique N-terminal domain. PTX3 is majorly present in monocytes, macrophages, fibroblasts, endothelial cells and adipocytes. It is an acute-phase protein mapped to human chromosome 3q25.32. |
| Immunogen: | recombinant human Pentraxin 3 protein expressed in HEK-293 cells |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |