Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal
SIGMA/SAB4200774 - clone 13C4, purified from hybridoma cell culture
Synonym: Anti-SLT-1 B subunit; Anti-SLT-1b; Anti-Shiga-like toxin 1 subunit B; Anti-Verocytotoxin 1 subunit B; Anti-Verotoxin 1 subunit B; Anti-stxB
Product Type: Chemical
Western Blotting Shiga Toxin 1, B subunit recombinant protein (Cat. No. SML0562) was separated on SDS-PAGE and probed with Anti Shiga Toxin 1, B subunit antibody, Mouse monoclonal, Clone: 13C4 (Cat. No. SAB4200774). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. Negative control: without first Antibody
Flow Cytometry 1×10E6 Ramos cells pretreated with Shiga Toxin 1, B subunit (Cat. No. SML0562) were labeled with 2.5 μg/test Anti-Shiga Toxin 1, B subunit antibody, Mouse monoclonal, Clone: 13C4 (Cat. No. SAB4200774) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without Shiga Toxin, Purple histogram: antibody specific staining.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 13C4, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| description | Research area: Microbiome |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~7 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | E. coli |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2.5-5 μg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit |
| immunoblotting: 1-2 μg/mL using purified recombinant Shiga Toxin 1-B subunit produced in E. coli (using PVDF membrane) | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | Q7BQ98  |
| Application: | Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal has been used in: • immunoblotting • flow cytometry • immunoprecipitation |
| Biochem/physiol Actions: | Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin. |
| Biochem/physiol Actions: | Shiga-toxin (Stx) produced by E. coli O157:H7 may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. All the Stx isoforms have similar structure and mechanism of action. The catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Gala(1-4)-Galb (1-4)-Glcb1-ceramide]. Gb3 is overexpressed in membranes of numerous tumor cells, Therefore, STxB binding to Gb3 receptors may be useful for cell-specific vectorization, labeling, and imaging purposes. |
| General description: | Monoclonal Anti-Shiga Toxin 1, B Subunit (mouse IgG1 isotype) is derived from the 13C4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a purified Shiga-like toxin from E. coli H30. Shiga toxins consist of two polypeptides, an A chain and a B chain non-covalently associate with an apparent stoichiometry of one A and five B chains to form the holotoxin. |
| General description: | The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. |
| Immunogen: | purified Shiga-like toxin from E.coli H30 |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |