Anti-Tubulin, Tyrosine antibody, Mouse monoclonal
SIGMA/SAB4200776 - clone TUB-1A2, purified from hybridoma cell culture
Synonym: Tubulin Alpha-1 Chain
Product Type: Chemical
Immunofluorescence Chicken Fibroblasts cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with 2 μg/mL Anti-Tubulin Tyrosine antibody, Mouse monoclonal, Clone: TUB-1A2 (Cat. No. SAB4200776). The antibody was developed using Goat Anti-Mouse IgG, FITC conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TUB-1A2, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG3 |
| mol wt | ~55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Xenopus, Arabidopsis sp., yeast, insect, rat, hamster, porcine, monkey, bovine, human, mouse, canine, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunofluorescence: 2-4 μg/mL using human HeLa or Chicken fibroblast cells | |
| immunohistochemistry: suitable | |
| UniProt accession no. | Q71U36  |
| Application: | Anti-Tubulin, Tyrosine antibody, mouse monoclonal may be used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Anti-Tubulin, Tyrosine antibody, Mouse monoclonal specifically recognizes the C-terminal Tyrosine of Tubulin protein. The antibody is non-reactive with cells that have been treated with pancreatic carboxypeptidase A under conditions which remove only the C-terminal tyrosine. Monoclonal Anti-Tubulin, Tyrosine recognizes Tubulin from human, mouse, chicken, bovine, rat, hamster, canine, monkey, porcine, arabidopsis, tobacco, yeast, Xenopus, insect, comb jelly, sponge, and worm origin. |
| Biochem/physiol Actions: | Tubulin is the major building block of microtubules which is essential for structural and mobility elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. It is also involved in the nervous system. Tubulin is associated with neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease. Mutations in the αβ- dimers and γ-tubulin are associated with malformation and impairment of cognitive disorders. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Tubulin, Tyrosine antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the TUB-1A2 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse. Tubulin is an intracellular cylindrical filamentous structure and is presented in eukaryotic cells. It consists of three isoforms α-, β- and γ-tubulin. Tubulin is a heterodimer of α- and β- tubulin subunits. |
| Immunogen: | a synthetic peptide containing the carboxy terminal amino acids of porcine a-tubulin |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |