Anti-Human IgM (μ-chain specific) antibody, Mouse monoclonal
SIGMA/SAB4200780 - clone MB-11, purified from hybridoma cell culture
Synonym: Anti-Human immunoglobulin M
Product Type: Chemical
ELISA Anti-Human IgM antibody, Mouse monoclonal, Clone: MB-11 (Cat. No. SAB4200780) was serially diluted to detect Human IgM by indirect ELISA. Reactivity is observed with only Human IgM but not with human IgG.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MB-11, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating |
| immunoblotting: suitable |
| Application: | The antibody is recommended to use in various immunological techniques, including ELISA and Immunoblot. |
| Biochem/physiol Actions: | Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal specifically recognizes m chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, k, l, and g chains. |
| Biochem/physiol Actions: | Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation. |
| General description: | Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgM protein. |
| General description: | Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocyte membranes. IgM is suggested to act as the first line of defense during microbial infections. IgM has a pentameric structure, in which monomers are linking together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation. |
| Immunogen: | Purified human IgM |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |