Anti-Haptoglobin antibody, Mouse monoclonal
SIGMA/SAB4200781 - clone HG-36, purified from hybridoma cell culture
Synonym: Anti-HP; Anti-Zonulin
Product Type: Chemical
ELISA Anti-Haptoglobin antibody, Mouse monoclonal, Clone: HG-36 (Cat. No. SAB4200781) was serially diluted to detect Haptoglobin (Cat. No. H3536) by indirect ELISA. Reactivity is observed with only haptoglobin but not with human IgG and α-1-acid-glycoprotein.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HG-36, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| ELISA: 0.2-0.4 μg/mL using 10 μg/ml human haptoglobin for coating | |
| immunoblotting: suitable | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | P00738  |
| Application: | The antibody is recommended to use in various immunological techniques, including ELISA, dot blot, immunoprecipitation, and immunoblot . The antibody reacts against native and denatured (non-reduced) human haptoglobin but does not recognize the haptoglobin under reducing conditions. |
| General description: | Haptoglobin, also known as HP, is a serum α2-glycoprotein that exists as a tetramer, composed of two smaller identical alpha (α) chains and two larger identical beta (β) chains. The α-chains are linked to each other by a disulfide bond and each β-chain is similarly linked to an α-chain. Monoclonal Anti-Haptoglobin specifically recognizes the three types of human haptoglobin (types 1-1, 2-1, and 2-2) The antibody recognizes both purified and haptoglobin in human serum, and shows no crossreactivity with human IgG, transferrin, C-reactive protein, α-1-acid glycoprotein, albumin, hemoglobin, or bovine, goat and sheep serum haptoglobin. |
| Immunogen: | purified haptoglobin from pooled human plasma |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |