Anti-Tenascin antibody, Mouse monoclonal
SIGMA/SAB4200782 - clone BC-24, purified from hybridoma cell culture
Synonym: Anti-Cytotactin; Anti-GMEM; Anti-GP 150-225; Anti-
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human tonsil sections with 5 μg/mL Anti-Tenascin antibody, Mouse monoclonal, Clone: BC-24 (Cat. No. SAB4200782) using Biotin/ExtrAvidin®-Peroxidase.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BC-24, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunoblotting: suitable | |
| immunohistochemistry: 5-10 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tonsil sections | |
| UniProt accession no. | P24821  |
| Application: | Anti-Tenascin antibody has been used: • in immunoblotting • in Immunohistochemistry • in flow cytometry • in enzyme linked immunosorbent assay(ELISA) • for blocking tenascin-C proliferating activity |
| Biochem/physiol Actions: | Tenascin-C (TN-C) functions in cell adhesion, fibroblast migration, and other processes related to tissue remodeling and wound healing. It has been proposed that actively growing, migrating and differentiating epithelial sheets can produce factors such as (Transforming growth factor beta) TGF-β to stimulate tenascin expression. Neo-expression or increased expression of tenascin has been found in the stroma of various tumors and during normal tissue repair. Intracytoplasmic tenascin immunoreactivity has been detected in malignant melanomas and in lung carcinomas, and it serves as a marker of stromal element proliferation in invasive breast carcinomas. High-molecular mass tenascin isoform plays a role in generating a permissive environment for proliferation, invasion, and metastasis of neoplastic epithelial cells. Human tenascin contains an Arg-Gly-Asp (RGD) sequence which may function in cell adhesion and mediates cell attachment through an RGD-dependent integrin receptor. |
| General description: | Monoclonal Anti-Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human tenascin. Tenascin-C (TN-C) is also known as Hexabrachion, Cytotactin, Neuronectin (NEC1). It is a high molecular mass extracellular matrix glycoprotein. Human tenascin is a disulfide-linked hexamer composed of 3 subunits of 190, 200, and 220 kDa. |
| Immunogen: | Human tenascin |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |