Anti-Human IgG2 antibody, Mouse monoclonal
SIGMA/SAB4200783 - clone HP-6014, purified from hybridoma cell culture
Synonym: Anti-Human immunoglobulin G2
Product Type: Chemical
ELISA Anti-Human IgG2 antibody, Mouse monoclonal, Clone: HP-6014 (Cat. No. SAB4200783) was serially diluted to detect Human IgG2 (Cat. No. I5279) by indirect ELISA.
ELISA Isotype specifity test: 0.15 μg/mL (red) or 0.3 μg/mL (blue) Anti-Human IgG2 antibody, Mouse monoclonal, Clone: HP-6014 (Cat. No. SAB4200783) were used to detect Human IgG2 (Cat. No. I5279). Reactivity is observed only with human IgG2 subclass (both Kappa and Lambda light chains) but not with human IgG1, IgG3 or IgG4.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HP-6014, monoclonal |
| concentration | ~1.0 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: 0.07-0.15 μg/mL using 1 μg/mL Human IgG2 myeloma for coating |
| Application: | Anti-Human IgG2 antibody, Mouse monoclonal may be used in immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunofluorescence, hemagglutination (HA) and hemagglutination inhibition. |
| Biochem/physiol Actions: | Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL) and an IgG1 response in the spleen. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes while IgG2 and IgG4 autoantibodies are much less efficient. The amount of the different IgG subclasses present in the blood shows variation with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is found in patients of ataxia telangiectasia. Low IgG2 levels were found in patients with Systemic lupus erythematosus (SLE) and juvenile diabetes mellitus. |
| General description: | Anti-Human IgG2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the HP-6014 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG2 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively. |
| Immunogen: | Purified human IgG2 myeloma protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |