Anti-Cellular Fibronectin antibody, Mouse monoclonal
SIGMA/SAB4200784 - clone FN-3E2, hybridoma cell culture supernatant
Synonym: Anti-CIG; Anti-Cold-insoluble globulin; Anti-FN
Product Type: Chemical
Immunofluorescence Hs68 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:2,000 Anti-Cellular Fibronectin antibody, Mouse monoclonal, Clone: FN-3E2 (Cat. No. SAB4200784). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of human Hs68 cells was separated on SDS-PAGE and probed with Anti-Cellular Fibronectin antibody, Mouse monoclonal, Clone: FN-3E2 (Cat. No. SAB4200784). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:200 2. Antibody dilution 1:500 3. Antibody dilution 1:1,000 4. Positive control 1:1,000 mouse Anti-Cellular Fibronectin antibody, Clone: FN-3E2 ascites fluid (Cat. No. F6140) 5. Negative control: without first Antibody
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FN-3E2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgM |
| mol wt | ~220-260 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunofluorescence: 1:2,000-1:4,000 using human foreskin fibroblast Hs68 cells | |
| immunohistochemistry: suitable | |
| UniProt accession no. | P02751  |
| Application: | Anti-Cellular Fibronectin antibody, Mouse monoclonal has been used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Application: | Anti-cellular Fibronectin antibody, mouse monocloneal has been used in various immunochemical techniques including immunoblotting (220-260 kDa), immunofluorescence, and immunohistochemistry. |
| Biochem/physiol Actions: | Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, haemostasis and thrombosis. It is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. |
| General description: | Anti-Cellular Fibronectin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the FN-3E2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the antigens released in culture from a breast cancer cell line. Fibronectin (FN), also known as cold-insoluble globulin (CIG), is an extracellular matrix multi-domain glycoprotein composed of two nearly identical disulphide bound polypeptides. Fibronectin 1 is a glycoprotein, that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Cellular fibronectin is produced by various cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells, and myocytes. |
| Immunogen: | Antigens released in culture from a breast cancer cell line |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. |
| Physical form: | The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains fetal calf serum. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |