Anti-Human IgG1 antibody, Mouse monoclonal
SIGMA/SAB4200786 - clone 8C/6-39, purified from hybridoma cell culture
Synonym: Anti-Human immunoglobulin G1
Product Type: Chemical
ELISA Anti-Human IgG1 antibody, Mouse monoclonal, Clone: 8c/6-39 (Cat. No. SAB4200786) was serially diluted to detect human IgG1 by indirect ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8C/6-39, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 3-6 μg/mL using Human IgG1 myeloma protein for coating. |
| Application: | Indirect ELISA: a working concentration of 3-6 μg/mL is recommended using 10 μg/mL Human IgG1 myeloma protein for coating. |
| General description: | Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial cells while IgG2 and IgG4 are far less efficient. The amount of the different IgG subclasses present in the bloodstream varies with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, a disproportionate elevation of IgG1 has been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders. |
| Immunogen: | The Fc fragment of a human IgG1 myeloma protein. |
| Other Notes: | In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |