Anti-phospho-GSK3B (pSer9) antibody produced in rabbit
SIGMA/SAB4300001 - affinity isolated antibody
Synonym: Anti-glycogen synthase kinase 3 beta antibody produced in rabbit
MDL Number: MFCD03455746
Product Type: Chemical
Immunohistochemistry Anti-phospho-GSK3B (pSer9): SAB4300001: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-GSK3B (pSer9) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-GSK3B (pSer9): SAB4300001: Staining of methanol-fixed HeLa cells showing cytoplasmic staining using Anti-phospho-GSK3B (pSer9) antibody.
Immunoblotting Anti-phospho-GSK3B (pSer9): SAB4300001: Analysis of extracts from 293 cells untreated (Lane 1) or treated with serum (Lane 2) using Anti-phospho-GSK3B (pSer9) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (T-T-SP-F-A) |
| isotype | IgG |
| mol wt | ~46 kDa |
| NCBI accession no. | NP_001139628.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer9) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P49841 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A), according to the protein GSK3B. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |