Anti-phospho-RELA (p65/pThr254) antibody produced in rabbit
SIGMA/SAB4300008 - affinity isolated antibody
Synonym: Anti-MGC131774 antibody produced in rabbit; Anti-NFKB3 antibody produced in rabbit; Anti-p65 antibody produced in rabbit; Anti-v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody produced in rabbit
MDL Number: MFCD00802848
Product Type: Chemical
Immunohistochemistry Anti-phospho-RELA (p65/pThr254): SAB4300008: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-RELA (p65/pThr254) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-RELA (p65/pThr254): SAB4300008: Staining of methanol-fixed HeLa using Anti-phospho-RELA (p65/pThr254) antibody.
Immunoblotting Anti-phospho-RELA (p65/pThr254): SAB4300008: Analysis of extracts from 293 cells untreated (Lane 1) or treated with TNF-α (Lane 2) using Anti-phospho-RELA (p65/pThr254) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (F-R-TP-P-P) |
| isotype | IgG |
| mol wt | ~65 kDa |
| NCBI accession no. | NP_001138610.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr254) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q04206 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P), according to the protein RELA. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |