Anti-phospho-STAT3 (pTyr705) antibody produced in rabbit
SIGMA/SAB4300033 - affinity isolated antibody
Synonym: Anti-APRF antibody produced in rabbit; Anti-FLJ20882 antibody produced in rabbit; Anti-HIES antibody produced in rabbit; Anti-MGC16063 antibody produced in rabbit; Anti-signal transducer and activator of transcription 3 (acute-phase response factor) antibody produced in rabbit
MDL Number: MFCD02097172
Product Type: Chemical
Immunohistochemistry Anti-phospho-STAT3 (pTyr705): SAB4300033: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-STAT3 (pTyr705) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunoblotting Anti-phospho-STAT3 (pTyr705): SAB4300033: Analysis of extracts from HeLa cells using Anti-phospho-STAT3 (pTyr705) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (A-P-YP-L-K) |
| isotype | IgG |
| mol wt | ~88 kDa |
| NCBI accession no. | NP_003141.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr705) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P40763 |
| Application: | Anti-phospho-STAT3 (pTyr705) antibody produced in rabbit has been used in immunohistochemical staining and immunoblotting. |
| Biochem/physiol Actions: | Signal transducer and activator of transcription 3 (STAT3) plays a vital role in various cellular responses such as induction of an acute-phase response in hepatoma cells, stimulation of proliferation in B lymphocytes, activation of terminal differentiation and growth arrest in monocytes, and regulation of the pluripotency of embryonic stem cells. Activated STAT3 has been involved in malignant transformation. STAT3 may be a key player in the pathogenesis of diverse human cancers which makes this molecule a prime target for novel therapies. Phospho-tyrosine residue 705 (Tyr705) Stat3 can be used as a potential marker for determining overall survival of node-negative breast cancer patients. Phosphorylated STAT3 might be involved in angiogenesis, anti-apoptosis and tumor progression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Signal transducer and activator of transcription 3 (STAT3) is a member of STAT family and it composed of 770 amino acids. The gene encoding protein consists of 24 exons and is extended over a length of 75170 bases on genomic DNA STAT3 is mapped on human chromosome 17q21.2. The protein is characterized with a conserved amino-terminal domain, a DNA-binding domain, an SH2 domain and a carboxy-terminal transactivation domain. |
| General description: | Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. |
| Immunogen: | Peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K), according to the protein STAT3. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |