Anti-phospho-STAT3 (pSer727) antibody produced in rabbit
SIGMA/SAB4300034 - affinity isolated antibody
Synonym: Anti-APRF antibody produced in rabbit; Anti-FLJ20882 antibody produced in rabbit; Anti-HIES antibody produced in rabbit; Anti-MGC16063 antibody produced in rabbit; Anti-signal transducer and activator of transcription 3 (acute-phase response factor) antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-phospho-STAT3 (pSer727): SAB4300034: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-STAT3 (pSer727) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-STAT3 (pSer727): SAB4300034: Staining of methanol-fixed HeLa cells using Anti-phospho-STAT3 (pSer727) antibody.
Immunoblotting Anti-phospho-STAT3 (pSer727): SAB4300034: Analysis of extracts from HeLa and HepG2 cells untreated or treated with PMA Anti-STAT3 (pSer727) antibody #SAB4300034. Anti-phospho-STAT3 (pSer727) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (P-M-SP-P-R) |
| isotype | IgG |
| mol wt | ~88 kDa |
| NCBI accession no. | NP_003141.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer727) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P40763 |
| Application: | Anti-phospho-STAT3 (pSer727) antibody produced in rabbit has been used in immunoblotting (1:1000) and immunofluorescence staining (1:200). |
| Biochem/physiol Actions: | Signal transducer and activator of transcription 3 (STAT3) protein play a key role in cell growth, apoptosis, metabolism, cellular respiration, and autophagy. It may act as a co-activator of signal transduction by glucocorticoid receptors. STAT3 regulates transcription. It is involved in the development of vertebrates and the regulation of inflammation and immunity. It might participate in airway inflammation, allergy, and asthma. Mutations in the STAT3 gene are associated with immunodeficiency, autoimmunity, and cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Signal transducer and activator of transcription 3 (STAT3), a constituent of the interleukin-6- (IL-6) activated acute phase response factor (APRF) complex, belongs to the signal transducer and activator of transcription (STAT) family. It is expressed in all types of tissues. The STAT3 gene with 24 exons is mapped to human chromosome 17q21.2. |
| General description: | Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R), according to the protein STAT3. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |