Anti-phospho-FOXO1 (pSer256) antibody produced in rabbit
SIGMA/SAB4300094 - affinity isolated antibody
Synonym: Anti-FKH1 antibody produced in rabbit; Anti-FKHR antibody produced in rabbit; Anti-FOXO1A antibody produced in rabbit; Anti-forkhead box O1 antibody produced in rabbit
MDL Number: MFCD03791549
Product Type: Chemical
Immunohistochemistry Anti-phospho-FOXO1 (pSer256): SAB4300094: Analysis of paraffin- embedded human breast carcinoma tissue using Anti-phospho-FOXO1 (pSer256) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-FOXO1 (pSer256): SAB4300094: Staining of methanol-fixed MCF7 cells using Anti-phospho-FOXO1 (pSer256) antibody (Red).
Immunoblotting Anti-phospho-FOXO1 (pSer256): SAB4300094: Analysis of extracts from 293 cells untreated or treated with serum using Anti-phospho-FOXO1 (pSer256) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (A-A-SP-M-D) |
| isotype | IgG |
| mol wt | 78-82 kDa |
| NCBI accession no. | NP_002006.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer256) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q12778 |
| Application: | Anti-phospho-FOXO1 (pSer256) antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | FOXO1 (forkhead box O1) blocks the differentiation of cell in fetal pancreatic progenitor cells. It controls the differentiation of adipocytes. FOXO1 is involved in the insulin regulation of glucose-6-phosphatase (G6p) expression. Additionally, this protein plays a vital role in tumor suppression by inducing growth arrest and apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | FOXO1 (forkhead box O1) is encoded by the gene mapped to human chromosome 13q14. The encoded protein is a transcription factor and is a member of the Foxo subfamily. |
| General description: | Transcription factor. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 256 (A-A-S(p)-M-D), according to the protein FOXO1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |