Anti-phospho-LIMK1 (pThr508) antibody produced in rabbit
SIGMA/SAB4300103 - affinity isolated antibody
Synonym: Anti-LIM domain kinase 1 antibody produced in rabbit; Anti-LIMK antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-phospho-LIMK1 (pThr508): SAB4300103: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-LIMK1 (pThr508) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-LIMK1 (pThr508): SAB4300103: Staining of methanol-fixed HeLa cells using Anti-phospho-LIMK1 (pThr508) antibody (Red).
Immunoblotting Anti-phospho-LIMK1 (pThr508): SAB4300103: Analysis of extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using Anti-phospho-LIMK1 (pThr508) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (R-Y-TP-V-V) |
| isotype | IgG |
| mol wt | ~72 kDa |
| NCBI accession no. | NP_002305.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr508) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P53667 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development. |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V), according to the protein LIMK1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |