Anti-phospho-RB1 (pSer780) antibody produced in rabbit
SIGMA/SAB4300109 - affinity isolated antibody
Synonym: Anti-OSRC antibody produced in rabbit; Anti-RB antibody produced in rabbit; Anti-p105-Rb antibody produced in rabbit; Anti-pRb antibody produced in rabbit; Anti-retinoblastoma 1 antibody produced in rabbit
MDL Number: MFCD00282455
Product Type: Chemical
Immunohistochemistry Anti-phospho-RB1 (pSer780): SAB4300109: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-RB1 (pSer780) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-RB1 (pSer780): SAB4300109: Staining of methanol-fixed HeLa cells using Anti-phospho-RB1 (pSer780) antibody (Red).
Immunoblotting Anti-phospho-RB1 (pSer780): SAB4300109: Analysis of extracts from 3T3 cells untreated or treated with UV using Anti-phospho-RB1 (pSer780) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (T-L-SP-P-I) |
| isotype | IgG |
| mol wt | ~110 kDa |
| NCBI accession no. | NP_000312.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer780) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P06400 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 780 (T-L-S(p)-P-I), according to the protein RB1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |